![SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (% SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%](https://cdn.numerade.com/ask_previews/6f0c47ee-38d7-4778-b4d8-d117b7d1affe_large.jpg)
SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
![Thermo Fisher on X: "PCR Tips, Tm Calculator and more on Platinum™ SuperFi™ DNA Polymerase https://t.co/s3AKNpZASK https://t.co/h3BEbxiTUv" / X Thermo Fisher on X: "PCR Tips, Tm Calculator and more on Platinum™ SuperFi™ DNA Polymerase https://t.co/s3AKNpZASK https://t.co/h3BEbxiTUv" / X](https://pbs.twimg.com/tweet_video_thumb/CiWJSg0UgAAjloD.jpg)
Thermo Fisher on X: "PCR Tips, Tm Calculator and more on Platinum™ SuperFi™ DNA Polymerase https://t.co/s3AKNpZASK https://t.co/h3BEbxiTUv" / X
![TP05 Primer design and melting temperature calculations for PCR primers - Primer design and melting - Studocu TP05 Primer design and melting temperature calculations for PCR primers - Primer design and melting - Studocu](https://d20ohkaloyme4g.cloudfront.net/img/document_thumbnails/48b5bfa14ef28a2d6166050c2f380fad/thumb_1200_1697.png)